Gluten must be in your system in order for testing to confirm celiac disease.

you better be consistently eating gluten for celiac disease testing to be accurate.

That’s becauseblood testsand intestinal tissue sample evaluations look for your body’s reaction to the protein.

Sliced wheat bread and strawberry jam jar

Dougal Waters/Getty Images

It’s recommended that anyone considering a gluten-free diet get tested for celiac disease first.

Without gluten in your system, celiac diseaseif presentcannot definitively be diagnosed.

Confirming celiac disease is important, as the diagnosis requires you to make diet changes to stay well.

It also puts you at higher risk of certain complications that need to be monitored for.

It will determine how strict you may need to be about reading labels.

That can be enough to trigger inflammation and symptoms if you have celiac.

Generally, it’s possible for you to reduce your risk of complications by following a strict gluten-free diet.

But you won’t know to do this without having undergone a gluten challenge to confirm celiac disease.

Gluten is, of course, not inherently harmful.

But in those with celiac disease, the body believes otherwise and treats it as such.

Antibodies need time to build in the bloodstream before they can be detected by blood tests.

you could have testing done, but the markers of celiac may simply not be there anymore.

Anendoscopy, which is a procedure to examine the small intestine, may not reveal any issues.

But some people have a condition known asIgA deficiencythat can cause false-negative results on antibody tests for celiac disease.

This allows them to look for damage and inflammation characteristic of celiac disease.

This rash is calleddermatitis herpetiformis.

This testing can be used to help diagnose both dermatitis herpetiformis and celiac disease.

you gotta be eating gluten in order for these tests to be accurate.

Such foods include pasta, cereal, and cookies.

Never undergo a gluten challenge without a healthcare provider’s guidance and oversight.

Always follow their specific instructions.

But even this isn’t always enough to accurately diagnose celiac in all cases.

In adults, between 50% and 100% will show positive test results within the same period.

How severe your symptoms might become over the course of your challenge is less clear.

There are also people whose overall health deteriorates dramatically over the course of a gluten challenge.

And some people might not notice any symptoms during the challenge at all.

However, there are two alternatives to undergoing a gluten challenge.

It only indicates your susceptibility to developing it.

This is a common decision for people who get severe symptoms from accidental gluten ingestion.

If you “cheat” and you do have celiac, you could be risking serious complications.

Another study found that interleukin-2 was the earliest and most reliable measurement in a two-week gluten challenge.

Larger studies are needed to confirm these findings and to develop guidelines for using this testing method.

Your healthcare provider may recommend a shorter or longer duration for the challenge depending on your symptoms.

The challenge typically runs about six to eight weeks.

Knowing this may be particularly helpful for loved ones who are ignoring their symptoms or putting off medical care.

Bruins MJ.The clinical response to gluten challenge: A review of the literature.Nutrients.

2013;5(11):46144641. doi:10.3390/nu5114614

American Society for Gastrointestinal Endoscopy.Celiac disease.

2013;108(5):656-676. doi:10.1038/ajg.2013.79

Celiac Disease Foundation.Diagnosis.

Massachusetts General Hospital.What to expect for your/your childs gluten challenge.

National Institute of Diabetes and Digestive and Kidney Diseases.Symptoms and causes of celiac disease.

2021;160(3):720-733.e8.